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Laser system allows high-resolution image generation

Posted: 19 May 2011 ?? ?Print Version ?Bookmark and Share

Keywords:fiber-based? laser? microscopy?

Mobius Photonics unveils a novel prototype laser source for super-resolution microscopy applications. Named 'Rainbow', the system is a fiber-based source of user-switchable, visible-wavelength laser pulses.

"Our Rainbow laser prototype is a testament to Mobius' competency in developing fiber-based solutions for the materials processing and life sciences markets," said Kiyomi Monro, Mobius Photonics CEO. "As Dr. Keaton, our chief scientist, presented in a paper at Photonics West, the Mobius technical team has developed a novel way of using fiber to generate visible wavelengths for stimulating the fluorescent markers as well as depleting fluorescence in super-resolution microscopy applications. Our initial design can generate three key wavelengths, and we have plans to extend that capability so that ultimately a Rainbow system could generate up to seven discrete wavelengths between 557C651nm. This laser may complement the capabilities offered by today's supercontinuum sources for microscopy."

The Rainbow prototype is a short-pulse, high-repetition-rate laser system that currently produces output at 585nm, 600nm and 616nm. The system's individual wavelengths can be switched on-the-fly, allowing users to tailor output to the fluorescent marker in a particular application. Rainbow features approximately 1ns pulses at repetition rates of up to 20MHz and pulse energies ranging from 25C50nJ per pulse, depending on the wavelength selected. The result is a laser system designed to allow high-resolution image generation with fast acquisition times.

Rainbow is air cooled and features a compact laser head to facilitate integration into microscopy systems. The laser's modular architecture is also intended to simplify integration, field servicing and system upgrades.

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